Coding

Part:BBa_K5452102:Design

Designed by: Bianca Henriques de Oliveira   Group: iGEM24_USP-EEL-Brazil   (2024-09-27)


Dicer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 570
    Illegal PstI site found at 844
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 844
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1751
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 570
    Illegal PstI site found at 844
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 570
    Illegal PstI site found at 844
    Illegal AgeI site found at 1536
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part had its sequence optimized for Saccharomyces cerevisiae


Source

This CDS can be found in the genome of the Naumovia castellii strain NRRL Y-12630

References